mouse anti-cask Search Results


mouse anti-cask  (NeuroMab)
90
NeuroMab mouse anti-cask
Mouse Anti Cask, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-cask/product/NeuroMab
Average 90 stars, based on 1 article reviews
mouse anti-cask - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mouse anti-cask  (Becton Dickinson)
90
Becton Dickinson mouse anti-cask
Expression of Nrxn-CTF inhibits presynaptic release. A, HA-Nrxn-CTF or HA-Nrxn-CTFΔPDZ were cotransfected with Synaptophysin-pHluorin (SypHy) in hippocampal neurons and stained with HA antibody. Presynaptic terminals expressing SypHy were detected with GFP fluorescence. B, Images showing SypHy response at 40 and 300 APs in axons expressing HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ. C, Peak amplitudes of SypHy fluorescence in axons transfected with HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ compared with control neurons (control synapses at 40 APs 1.0 ± 0.060; HA-Nrxn-CTF synapses at 40 APs 0.411 ± 0.023; HA-Nrxn-CTFΔPDZ synapses at 40 APs 0.697 ± 0.041; control synapses at 300 APs 3.580 ± 0.178; HA-Nrxn-CTF synapses at 300 APs 0.980 ± 0.059; HA-Nrxn-CTFΔPDZ synapses at 300 APs 1.949 ± 0.085, ***p < 0.001, Kruskal–Wallis followed by the post hoc Dunn's test). D, Immunofluorescence experiments with HA and <t>CASK</t> <t>antibodies</t> showing CASK accumulation at HA-Nrxn-CTF expressing axons. E, Quantification of CASK staining (normalized CASK area in transfected axons: GFP 1.0 ± 0.079; HA-Nrxn-CTF 2.636 ± 0.107; HA-Nrxn-CTFΔPDZ 1.717 ± 0.113; ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Normalized CASK intensity in transfected axons: GFP 1.0 ± 0.010; HA-Nrxn-CTF 1.365 ± 0.028, HA-Nrxn-CTFΔPDZ 1.081 ± 0.015, *p < 0.05, ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Data are shown from 26–31 images from two independent experiments from different cultures. Scale bars, 5 μm. Error bars indicate SEM.
Mouse Anti Cask, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-cask/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-cask - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
anti-rat cask/lin2 mouse monoclonal antibody #75-000  (NeuroMab)
90
NeuroMab anti-rat cask/lin2 mouse monoclonal antibody #75-000
Expression of Nrxn-CTF inhibits presynaptic release. A, HA-Nrxn-CTF or HA-Nrxn-CTFΔPDZ were cotransfected with Synaptophysin-pHluorin (SypHy) in hippocampal neurons and stained with HA antibody. Presynaptic terminals expressing SypHy were detected with GFP fluorescence. B, Images showing SypHy response at 40 and 300 APs in axons expressing HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ. C, Peak amplitudes of SypHy fluorescence in axons transfected with HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ compared with control neurons (control synapses at 40 APs 1.0 ± 0.060; HA-Nrxn-CTF synapses at 40 APs 0.411 ± 0.023; HA-Nrxn-CTFΔPDZ synapses at 40 APs 0.697 ± 0.041; control synapses at 300 APs 3.580 ± 0.178; HA-Nrxn-CTF synapses at 300 APs 0.980 ± 0.059; HA-Nrxn-CTFΔPDZ synapses at 300 APs 1.949 ± 0.085, ***p < 0.001, Kruskal–Wallis followed by the post hoc Dunn's test). D, Immunofluorescence experiments with HA and <t>CASK</t> <t>antibodies</t> showing CASK accumulation at HA-Nrxn-CTF expressing axons. E, Quantification of CASK staining (normalized CASK area in transfected axons: GFP 1.0 ± 0.079; HA-Nrxn-CTF 2.636 ± 0.107; HA-Nrxn-CTFΔPDZ 1.717 ± 0.113; ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Normalized CASK intensity in transfected axons: GFP 1.0 ± 0.010; HA-Nrxn-CTF 1.365 ± 0.028, HA-Nrxn-CTFΔPDZ 1.081 ± 0.015, *p < 0.05, ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Data are shown from 26–31 images from two independent experiments from different cultures. Scale bars, 5 μm. Error bars indicate SEM.
Anti Rat Cask/Lin2 Mouse Monoclonal Antibody #75 000, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-rat cask/lin2 mouse monoclonal antibody #75-000/product/NeuroMab
Average 90 stars, based on 1 article reviews
anti-rat cask/lin2 mouse monoclonal antibody #75-000 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mouse monoclonal anti-cask antibody  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc mouse monoclonal anti-cask antibody
Expression of Nrxn-CTF inhibits presynaptic release. A, HA-Nrxn-CTF or HA-Nrxn-CTFΔPDZ were cotransfected with Synaptophysin-pHluorin (SypHy) in hippocampal neurons and stained with HA antibody. Presynaptic terminals expressing SypHy were detected with GFP fluorescence. B, Images showing SypHy response at 40 and 300 APs in axons expressing HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ. C, Peak amplitudes of SypHy fluorescence in axons transfected with HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ compared with control neurons (control synapses at 40 APs 1.0 ± 0.060; HA-Nrxn-CTF synapses at 40 APs 0.411 ± 0.023; HA-Nrxn-CTFΔPDZ synapses at 40 APs 0.697 ± 0.041; control synapses at 300 APs 3.580 ± 0.178; HA-Nrxn-CTF synapses at 300 APs 0.980 ± 0.059; HA-Nrxn-CTFΔPDZ synapses at 300 APs 1.949 ± 0.085, ***p < 0.001, Kruskal–Wallis followed by the post hoc Dunn's test). D, Immunofluorescence experiments with HA and <t>CASK</t> <t>antibodies</t> showing CASK accumulation at HA-Nrxn-CTF expressing axons. E, Quantification of CASK staining (normalized CASK area in transfected axons: GFP 1.0 ± 0.079; HA-Nrxn-CTF 2.636 ± 0.107; HA-Nrxn-CTFΔPDZ 1.717 ± 0.113; ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Normalized CASK intensity in transfected axons: GFP 1.0 ± 0.010; HA-Nrxn-CTF 1.365 ± 0.028, HA-Nrxn-CTFΔPDZ 1.081 ± 0.015, *p < 0.05, ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Data are shown from 26–31 images from two independent experiments from different cultures. Scale bars, 5 μm. Error bars indicate SEM.
Mouse Monoclonal Anti Cask Antibody, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-cask antibody/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
mouse monoclonal anti-cask antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Expression of Nrxn-CTF inhibits presynaptic release. A, HA-Nrxn-CTF or HA-Nrxn-CTFΔPDZ were cotransfected with Synaptophysin-pHluorin (SypHy) in hippocampal neurons and stained with HA antibody. Presynaptic terminals expressing SypHy were detected with GFP fluorescence. B, Images showing SypHy response at 40 and 300 APs in axons expressing HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ. C, Peak amplitudes of SypHy fluorescence in axons transfected with HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ compared with control neurons (control synapses at 40 APs 1.0 ± 0.060; HA-Nrxn-CTF synapses at 40 APs 0.411 ± 0.023; HA-Nrxn-CTFΔPDZ synapses at 40 APs 0.697 ± 0.041; control synapses at 300 APs 3.580 ± 0.178; HA-Nrxn-CTF synapses at 300 APs 0.980 ± 0.059; HA-Nrxn-CTFΔPDZ synapses at 300 APs 1.949 ± 0.085, ***p < 0.001, Kruskal–Wallis followed by the post hoc Dunn's test). D, Immunofluorescence experiments with HA and CASK antibodies showing CASK accumulation at HA-Nrxn-CTF expressing axons. E, Quantification of CASK staining (normalized CASK area in transfected axons: GFP 1.0 ± 0.079; HA-Nrxn-CTF 2.636 ± 0.107; HA-Nrxn-CTFΔPDZ 1.717 ± 0.113; ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Normalized CASK intensity in transfected axons: GFP 1.0 ± 0.010; HA-Nrxn-CTF 1.365 ± 0.028, HA-Nrxn-CTFΔPDZ 1.081 ± 0.015, *p < 0.05, ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Data are shown from 26–31 images from two independent experiments from different cultures. Scale bars, 5 μm. Error bars indicate SEM.

Journal: The Journal of Neuroscience

Article Title: Proteolytic Processing of Neurexins by Presenilins Sustains Synaptic Vesicle Release

doi: 10.1523/JNEUROSCI.1357-17.2017

Figure Lengend Snippet: Expression of Nrxn-CTF inhibits presynaptic release. A, HA-Nrxn-CTF or HA-Nrxn-CTFΔPDZ were cotransfected with Synaptophysin-pHluorin (SypHy) in hippocampal neurons and stained with HA antibody. Presynaptic terminals expressing SypHy were detected with GFP fluorescence. B, Images showing SypHy response at 40 and 300 APs in axons expressing HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ. C, Peak amplitudes of SypHy fluorescence in axons transfected with HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ compared with control neurons (control synapses at 40 APs 1.0 ± 0.060; HA-Nrxn-CTF synapses at 40 APs 0.411 ± 0.023; HA-Nrxn-CTFΔPDZ synapses at 40 APs 0.697 ± 0.041; control synapses at 300 APs 3.580 ± 0.178; HA-Nrxn-CTF synapses at 300 APs 0.980 ± 0.059; HA-Nrxn-CTFΔPDZ synapses at 300 APs 1.949 ± 0.085, ***p < 0.001, Kruskal–Wallis followed by the post hoc Dunn's test). D, Immunofluorescence experiments with HA and CASK antibodies showing CASK accumulation at HA-Nrxn-CTF expressing axons. E, Quantification of CASK staining (normalized CASK area in transfected axons: GFP 1.0 ± 0.079; HA-Nrxn-CTF 2.636 ± 0.107; HA-Nrxn-CTFΔPDZ 1.717 ± 0.113; ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Normalized CASK intensity in transfected axons: GFP 1.0 ± 0.010; HA-Nrxn-CTF 1.365 ± 0.028, HA-Nrxn-CTFΔPDZ 1.081 ± 0.015, *p < 0.05, ***p < 0.001, one-way ANOVA followed by the post hoc Bonferroni's test. Data are shown from 26–31 images from two independent experiments from different cultures. Scale bars, 5 μm. Error bars indicate SEM.

Article Snippet: The following primary antibodies were used: rat anti-HA (1:1000; clone 3F10; Roche; RRID: AB_390918 ), mouse anti-CASK (1:200; BD Transduction Lab; RRID: AB_398103 ) and rabbit anti-VGluT1 (1:1500; Synaptic Systems; RRID: AB_887877 ).

Techniques: Expressing, Staining, Fluorescence, Transfection, Immunofluorescence

Distribution of synaptic markers at synapses induced by Nlgn1 in hippocampal neurons with impaired PS function. A–C, Immunofluorescence staining and quantifications of the synaptic vesicle-resident marker Synaptophysin-pHluorin (SypHy) and Nlgn1 in PS cDKO hippocampal neurons (normalized SypHy area: control 1.0 ± 0.105; control ΔCre 1.103 ± 0.076; PS cDKO 0.992 ± 0.073, p > 0.05, one-way ANOVA followed by the post hoc Bonferroni's test; normalized SypHy intensity: control 1.0 ± 0.025; control ΔCre 0.986 ± 0.043; PS cDKO 0.921 ± 0.050, p > 0.05, Kruskal–Wallis followed by the post hoc Dunn's test; normalized Nlgn1 intensity: control 1.0 ± 0.095; control ΔCre 0.902 ± 0.060; PS cDKO 1.004 ± 0.041, p > 0.05, Kruskal–Wallis followed by the post hoc Dunn's test). Nlgn1 was detected with an HA antibody. Only Nlgn1 intensity was analyzed due to the high dendritic area covered by overexpressed Nlgn1. D–F, Immunofluorescence staining of SypHy and Nlgn1 and quantifications in hippocampal neurons incubated with DAPT (normalized SypHy area: control 1.0 ± 0.035; DAPT 0.896 ± 0.044, p = 0.076, Student's t test; normalized SypHy intensity: control 1.0 ± 0.017; DAPT 1.004 ± 0.022, p = 0.878, Student's t test; normalized Nlgn1 intensity: control 1.0 ± 0.040; DAPT 1.046 ± 0.034, p = 0.402. Mann–Whitney U test). G–I, Immunofluorescence signal of SypHy and Nlgn1 and quantifications in hippocampal neurons expressing PS1 D385A (normalized SypHy area: control 1.0 ± 0.076; PS1 D385A 0.825 ± 0.058, p = 0.075, Student's t test; normalized SypHy intensity: control 1.0 ± 0.011; PS1 D385A 0.966 ± 0.019, p = 0.133, Student's t test; normalized Nlgn1 intensity: control 1.0 ± 0.034; PS1 D385A 1.029 ± 0.031, p = 0.538, Student's t test). J, Immunofluorescence experiments with CASK and cyto-Nrxn antibodies showing increased signals at Nlgn1 synapses of PS cDKO neurons. K, L, Graphs showing quantifications of the relative area and mean intensity of cyto-Nrxn (K) and CASK (L) fluorescent signals at Nlgn1 synapses of control (ΔCre) and PS cDKO neurons (normalized Nrxn area: control ΔCre 1.0 ± 0.132; PS cDKO 1.716 ± 0.153, p = 0.001, Student's t test; normalized Nrxn intensity: control ΔCre 1.0 ± 0.015; PS cDKO, 1.061 ± 0.022, p = 0.029, Mann–Whitney U test; normalized CASK area: control ΔCre 1.0 ± 0.109; PS cDKO 1.869 ± 0.178, p = 0.0002, Mann–Whitney U test; normalized CASK intensity: control ΔCre 1.0 ± 0.021; PS cDKO 1.094 ± 0.031, p = 0.033, Mann–Whitney U test). Data from 15–25 experiments were obtained from two to three independent cultures. Scale bars, 5 μm. *p < 0.05, ***p < 0.001. Error bars indicate SEM.

Journal: The Journal of Neuroscience

Article Title: Proteolytic Processing of Neurexins by Presenilins Sustains Synaptic Vesicle Release

doi: 10.1523/JNEUROSCI.1357-17.2017

Figure Lengend Snippet: Distribution of synaptic markers at synapses induced by Nlgn1 in hippocampal neurons with impaired PS function. A–C, Immunofluorescence staining and quantifications of the synaptic vesicle-resident marker Synaptophysin-pHluorin (SypHy) and Nlgn1 in PS cDKO hippocampal neurons (normalized SypHy area: control 1.0 ± 0.105; control ΔCre 1.103 ± 0.076; PS cDKO 0.992 ± 0.073, p > 0.05, one-way ANOVA followed by the post hoc Bonferroni's test; normalized SypHy intensity: control 1.0 ± 0.025; control ΔCre 0.986 ± 0.043; PS cDKO 0.921 ± 0.050, p > 0.05, Kruskal–Wallis followed by the post hoc Dunn's test; normalized Nlgn1 intensity: control 1.0 ± 0.095; control ΔCre 0.902 ± 0.060; PS cDKO 1.004 ± 0.041, p > 0.05, Kruskal–Wallis followed by the post hoc Dunn's test). Nlgn1 was detected with an HA antibody. Only Nlgn1 intensity was analyzed due to the high dendritic area covered by overexpressed Nlgn1. D–F, Immunofluorescence staining of SypHy and Nlgn1 and quantifications in hippocampal neurons incubated with DAPT (normalized SypHy area: control 1.0 ± 0.035; DAPT 0.896 ± 0.044, p = 0.076, Student's t test; normalized SypHy intensity: control 1.0 ± 0.017; DAPT 1.004 ± 0.022, p = 0.878, Student's t test; normalized Nlgn1 intensity: control 1.0 ± 0.040; DAPT 1.046 ± 0.034, p = 0.402. Mann–Whitney U test). G–I, Immunofluorescence signal of SypHy and Nlgn1 and quantifications in hippocampal neurons expressing PS1 D385A (normalized SypHy area: control 1.0 ± 0.076; PS1 D385A 0.825 ± 0.058, p = 0.075, Student's t test; normalized SypHy intensity: control 1.0 ± 0.011; PS1 D385A 0.966 ± 0.019, p = 0.133, Student's t test; normalized Nlgn1 intensity: control 1.0 ± 0.034; PS1 D385A 1.029 ± 0.031, p = 0.538, Student's t test). J, Immunofluorescence experiments with CASK and cyto-Nrxn antibodies showing increased signals at Nlgn1 synapses of PS cDKO neurons. K, L, Graphs showing quantifications of the relative area and mean intensity of cyto-Nrxn (K) and CASK (L) fluorescent signals at Nlgn1 synapses of control (ΔCre) and PS cDKO neurons (normalized Nrxn area: control ΔCre 1.0 ± 0.132; PS cDKO 1.716 ± 0.153, p = 0.001, Student's t test; normalized Nrxn intensity: control ΔCre 1.0 ± 0.015; PS cDKO, 1.061 ± 0.022, p = 0.029, Mann–Whitney U test; normalized CASK area: control ΔCre 1.0 ± 0.109; PS cDKO 1.869 ± 0.178, p = 0.0002, Mann–Whitney U test; normalized CASK intensity: control ΔCre 1.0 ± 0.021; PS cDKO 1.094 ± 0.031, p = 0.033, Mann–Whitney U test). Data from 15–25 experiments were obtained from two to three independent cultures. Scale bars, 5 μm. *p < 0.05, ***p < 0.001. Error bars indicate SEM.

Article Snippet: The following primary antibodies were used: rat anti-HA (1:1000; clone 3F10; Roche; RRID: AB_390918 ), mouse anti-CASK (1:200; BD Transduction Lab; RRID: AB_398103 ) and rabbit anti-VGluT1 (1:1500; Synaptic Systems; RRID: AB_887877 ).

Techniques: Immunofluorescence, Staining, Marker, Incubation, MANN-WHITNEY, Expressing

Processing of HA-Nrxn-CTF proteins by PS. A, Schematic drawings showing HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ constructs compared with Nrxn1-β. PDZ labels the last three residues that form a PDZ-binding domain. B, Western blot of transfected HEK293T cells in the presence of DAPT. C, Lysates of hippocampal neurons infected with HA-Nrxn1-β, HA-Nrxn-CTF or HA-Nrxn-CTFΔPDZ and incubated with DAPT were immunoblotted with HA and cyto-Nrxn antibodies, as indicated. The decreased mobility observed for HA-Nrxn-CTF compared with Nrxn-CTF is likely due to the insertion of the HA tag. D, Lysates and HA immunoprecipitates of HEK293T cells coexpressing CASK and empty vector, HA-Nrxn-CTF, or HA-Nrxn-CTFΔPDZ. HA-Nrxn1-β was coexpressed as a positive control. The isolated HA complexes were immunoblotted with CASK and HA antibodies, as indicated. E, Hippocampal neurons expressing HA-Nrxn-CTF, HA-Nrxn-CTFΔPDZ, or HA-Nrxn1-β by lentiviral infections were immunoprecipitated with HA antibodies and the presence of CASK and HA proteins analyzed by Western blot.

Journal: The Journal of Neuroscience

Article Title: Proteolytic Processing of Neurexins by Presenilins Sustains Synaptic Vesicle Release

doi: 10.1523/JNEUROSCI.1357-17.2017

Figure Lengend Snippet: Processing of HA-Nrxn-CTF proteins by PS. A, Schematic drawings showing HA-Nrxn-CTF and HA-Nrxn-CTFΔPDZ constructs compared with Nrxn1-β. PDZ labels the last three residues that form a PDZ-binding domain. B, Western blot of transfected HEK293T cells in the presence of DAPT. C, Lysates of hippocampal neurons infected with HA-Nrxn1-β, HA-Nrxn-CTF or HA-Nrxn-CTFΔPDZ and incubated with DAPT were immunoblotted with HA and cyto-Nrxn antibodies, as indicated. The decreased mobility observed for HA-Nrxn-CTF compared with Nrxn-CTF is likely due to the insertion of the HA tag. D, Lysates and HA immunoprecipitates of HEK293T cells coexpressing CASK and empty vector, HA-Nrxn-CTF, or HA-Nrxn-CTFΔPDZ. HA-Nrxn1-β was coexpressed as a positive control. The isolated HA complexes were immunoblotted with CASK and HA antibodies, as indicated. E, Hippocampal neurons expressing HA-Nrxn-CTF, HA-Nrxn-CTFΔPDZ, or HA-Nrxn1-β by lentiviral infections were immunoprecipitated with HA antibodies and the presence of CASK and HA proteins analyzed by Western blot.

Article Snippet: The following primary antibodies were used: rat anti-HA (1:1000; clone 3F10; Roche; RRID: AB_390918 ), mouse anti-CASK (1:200; BD Transduction Lab; RRID: AB_398103 ) and rabbit anti-VGluT1 (1:1500; Synaptic Systems; RRID: AB_887877 ).

Techniques: Construct, Binding Assay, Western Blot, Transfection, Infection, Incubation, Plasmid Preparation, Positive Control, Isolation, Expressing, Immunoprecipitation